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KMID : 0378019920350100033
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New Medical Journal
1992 Volume.35 No. 10 p.33 ~ p.46
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Electron Microscopic Cytochemical Study of Resorptive Functions of Bone Cells Following Parathyroid Transplatation
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Abstract
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An electron microscopic cytochemical study was carried out to investigate the precise mechanisms for absorption of organic bone matrix components during bone reoption induced by parathyroid hormone. Transplantation` of the parathyroid tissue was done into
the medullary cavity of the tibia in the rats, which were examined 1, 2, and 4, days after the operation, employing horseradish peroxidase as a soluble protein tracer. The tibial bone were extirpated and examined by scanning and transmission election microscopy with cytochemical procedures.
The results obtained were summarized as follows.
Intravenously-injected peroxidase was filled the osteoid layer and penetrated into the osteocyte lacunae and canaliculi, but did not enter the mineralized bone matrix. Whereas bone cells of resting state rarely took up exogenous peroxidase, activated osteoblasts and osteoclasts treated with parathyroil hormone actively endocytosed peroxidase in pinocytotic coated vesicles, tubular structures and vacuoles. They also formed endocytotic vacuoles contatining peroxidase in the Golgi area. The osteocytes treated with parathyroid hormone did not changed endocytotic activity.
The results strongly suggest that both osteoblasts and osteoclasts are to degrade and resorb bone matrix organic components during resorption induced by parathyroid hormone.
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